Characterization of the domestic goat ?? T cell receptor gene loci and gene usage

Goats and cattle diverged 30 million years ago but retain similarities in immune system genes. Here, the caprine T cell receptor (TCR) gene loci transcription of its genes were examined compared to cattle. We annotated TCR using an improved genome assembly (ARS1) a highly homozygous San Clemente goat. This has already proven useful for describing other including antibody leucocyte receptors. Both TCR? (TRG) TCR? (TRD) similarly organized goats as sequences conserved. However, number varied slightly result duplications differences occurred mutations resulting pseudogenes. WC1+ ?? cells have been shown use from only one six available cassettes. The structure that C? product is unique may be necessary interact with WC1 signal transduction following antigen ligation. Using RT-PCR PacBio sequencing, we observed same restriction goat cells. In contrast, WC1? populations had diverse usage although propensity particular differed between two populations. Noncanonical recombination (RSS) largely correlated restricted expression ? Finally, found incorporate multiple TRD diversity single transcript, unusual feature among mammals also previously